Isolation of human fibroadipogenic progenitors and satellite cells from frozen muscle biopsies

Skeletal muscle contains multiple cell types that work together to maintain tissue homeostasis. Among these, satellite cells (SC) and fibroadipogenic progenitors (FAPs) are the two main stem pools. Studies of these using animal models have shown importance interactions between in repair healthy muscle, degeneration dystrophic muscle. Due unavailability fresh patient biopsies, similar analysis human FAPs SCs is limited especially among muscular dystrophy patients. To address this issue here we describe a method allows use frozen skeletal biopsies simultaneously isolate grow from or We show while purified differentiate into mature myotubes, can adipocytes fibroblasts demonstrating their multipotency. find be immortalized (iFAPs) retain These approaches open door for carrying out personalized with lead loss.